A mysterious young drifter who calls herself Nomi Malone hitches a ride to Las Vegas, Nevada, and begins working as a strip club dancer, and sets about clawing her way to the top of the Vegas showgirls.

When drag queen Anthony (Hugo Weaving) agrees to take his act on the road, he invites friends Adam (Guy Pearce) and Bernadette (Terence Stamp) to come along. In their colorful bus, named Priscilla, the three performers travel across the Australian desert performing for enthusiastic crowds and homophobic locals.

A gender-queer punk-rock singer from East Berlin tours the U.S. with her band as she tells her life story and follows the former lover/band-mate who stole her songs.